| 酵母静息细胞催化丙酮酸乙酯不对称还原制(S)-乳酸乙酯 |
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| 引用本文： | 王丹,张强,李旺,戚南昌,郭春晓,杨志荣,张杰. 酵母静息细胞催化丙酮酸乙酯不对称还原制(S)-乳酸乙酯[J]. 催化学报, 2011, 32(6): 1035-1039. DOI: 10.3724/SP.J.1088.2011.01236 |
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| 作者姓名： | 王丹 张强 李旺 戚南昌 郭春晓 杨志荣 张杰 |
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| 作者单位： | 四川大学生命科学学院;成都医学院生物医学系;成都医学院医学检验系; |
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| 基金项目： | 四川省科技厅项目(2010GZ0164)~~ |
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| 摘 要： | 从污水处理池及其附近土壤中分离到36株可将丙酮酸乙酯不对称还原成(S-乳酸乙酯的菌株,经多次复筛,最终得到一株具有较高催化活性的酵母菌BTY18-6.以BTY18-6的静息细胞为催化剂,在水相中进行丙酮酸乙酯不对称还原成(S)-乳酸乙酯的反应,并对反应条件进行了优化.结果表明,以2.5%葡萄糖为辅助底物,反应体系初始p...
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| 关 键 词： | 酵母静息细胞 生物催化 丙酮酸乙酯 不对称还原 (S)-乳酸乙酯 |
Asymmetric Reduction of Ethyl Pyruvate Catalyzed by Yeast Resting Cells to (S)-Ethyl Lactate |
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| WANG Dan,ZHANG Qiang,LI Wang,QI Nanchang,GUO Chunxiao,YANG Zhirong,ZHANG Jie. Asymmetric Reduction of Ethyl Pyruvate Catalyzed by Yeast Resting Cells to (S)-Ethyl Lactate[J]. Chinese Journal of Catalysis, 2011, 32(6): 1035-1039. DOI: 10.3724/SP.J.1088.2011.01236 |
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| Authors: | WANG Dan ZHANG Qiang LI Wang QI Nanchang GUO Chunxiao YANG Zhirong ZHANG Jie |
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| Affiliation: | WANG Dan1,2,ZHANG Qiang2,LI Wang2,QI Nanchang3,GUO Chunxiao1,YANG Zhirong1,ZHANG Jie1,* 1Life Science College,Sichuan University,Chengdu 610065,Sichuan,China 2Department of Biomedicine,Chengdu Medical College,Chengdu 610083,China 3Department of Medical Examination,China |
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| Abstract: | Thirty six strains that exhibited the catalytic activity for asymmetric reduction of ethyl pyruvate to (S)-ethyl lactate were isolated from sewage of a chemical factory in Chengdu,China and the neighboring soil.Among them,yeast strain BTY18-6 has high catalytic activity.The asymmetric reduction of ethyl pyruvate to (S)-ethyl lactate catalyzed by BTY18-6 resting cells in an aqueous phase was examined,and the parameters influencing the bio-reduction were optimized.The results showed that,under the optimized c... |
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| Keywords: | yeast resting cell biological catalysis ethyl pyruvate asymmetric reduction (S)-ethyl lactate |
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