Determination of grepafloxacin in plasma samples by HPLC: Application to clinical pharmacokinetic studies |
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Authors: | S Perez-Olivan C Solans M A García I Pinilla F Honrubia M A Bregante |
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Institution: | (1) Department of Ophthalmology, Miguel Servet University Hospital, Isabel La Catolica 1-3, 50009 Zaragoza, Spain;(2) Department of Analytical Chemistry, Veterinary Faculty, University of Zaragoza, Miguel Servet 177, 50013 Zaragoza, Spain;(3) Department of Pharmacology and Physiology, Veterinary Faculty, University of Zaragoza, Miguel Servet 177, 50013 Zaragoza, Spain |
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Abstract: | Summary A sensitive HPLC assay for the determination of grepafloxacin (GRE) in biological samples is described. Sample preparations
were carried out by adding phosphate buffer (pH 7.4, 0.1M), followed by extraction with trichloromethane. GRE and the internal
standard, enrofloxacin (ENR), were separated on a reversed-phase column using an aqueous phosphate solution-acetonitrile (78∶22)
mobile phase. The concentrations of ENR and GRE eluting of the column with retention times of 2.55, and 4.90 min, respectively
were monitored by fluorescence atλ
ex 338 andλ
em 425 nm. The method was shown to be linear from 5 to 4000 ng mL−1. The detection and quantitation limits were 5 and 10 ng mL−1, respectively. Mean recovery was determined as 90%. Inter- and intra-assay precisions were 3.0% and 3.5% respectively. The
method was applied to the determination of GRE in plasma samples collected during clinical pharmacokinetic studies. |
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Keywords: | Column liquid chromatography Grepafloxacin Pharmacokinetics |
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