A new macrocyclic ligand, L was synthesized using the high dilution condition with condensation of triethylene glycol diamine and terephtalaldehyde in ethanol. The obtained product, L was identified by FT‐IR, 1H‐NMR, 13C‐NMR and Mass spectroscopy. The extraction equilibrium constants were estimated using dichloromethane/water membranes transfer with ICP‐AES and AES spectroscopy. Biological studies of this compound was determinated with disc diffusion method. The biological activity results showed that the synthesized ligand L has high activity against the studied microorganisms and high complexation ability against the Fe2+ cation.
