几种寄生虫抗原组分的SDS-PAGE电泳比较分析

通过提取制备猪带绦虫、猪囊尾蚴虫体、猪囊虫囊液、多房型束球绦虫、肝片吸虫、细颈囊尾蚴、细颈囊尾蚴囊液和羊绦虫等8种寄生虫抗原，运用SDS－PAGE电泳法比较各种抗原的蛋白质的组成，不连续SDS－PAGE电泳结果显示猪带绦虫、猪囊尾蚴虫体、猪囊虫囊液、多房示绦虫、肝片吸虫、细颈囊尾蚴、细颈囊尾蚴囊液和羊绦虫等几种抗原的蛋白质在凝胶中分别有31，23，18，17，21，22，8和24条带，分子量在13kD-134kD之间，8种抗原的电泳带之间既有相似性又有特异性，其抗原的共同蛋白质亚基带的分子量为63kD,38kD和24kD。

Studies On Antigens of Several Parasites by SDS-PAGE Protein Electrophoresis

Eight antigens of several parasites cysticercus cellulosae fluid (CCF), Cysticercus cellulosae worm (CCW), Taenia solium antigen (TS), Echinococcus mutilocyulavis (EM), Fasciola hepatica (FH), Cysticercus tenuicollis (CT), Cysticercus tenuicollis fluid (CTF) and Moniezia expansa (ME). obtained through different extractive methods. Molecular weight(M.W.) of the antigens were analyzed by discontinuous SDS PAGE. Discontinuous SDS PAGE by using seperated gel of 10% indicated that M.W.of antigens of several parasites were between 13kD and 133kD. Ag CCF, Ag CCW, Ag TS, Ag EM, Ag FH, Ag CT, Ag CTF and Ag ME contain bands: 31, 23, 18, 17, 21, 22, 8 and 24, respectively. These bands have not only similarity but also specificity. The results showed that the main same antigenic bands among the eight kinds of antigens were 63kD, 38kD and 24kD. The results of the experiment can provide scientific basis for clinical diagnosis and preparation of protective vaccine of the parasites.