On the carrier properties of perfluoropolyether-betaine mixed vesicles: the contribution of electron spin resonance spectroscopy |
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Authors: | Giacomo Martini Manuela Balzi Sandra Ristori |
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Affiliation: | a Department of Chemistry, University of Florence, Polo Scientifico, Via della Lastruccia 3, 50019 Sesto F.no, Firenze, Italy b Radiobiology Laboratory, Department of Clinical Physiopathology, University of Florence, 50134 Firenze, Italy |
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Abstract: | The carrier properties of mixed unilamellar vesicles of fluorocarbon-hydrocarbon surfactants built up with the ammonium salt of a perfluoropolyether and n-dodecylbetaine were investigated by electron spin resonance spectroscopy (ESR). The ESR-active lipophilic nitroxide 5-doxylstearic acid (5-DSA) was used as a model of a lipophilic drug to be carried and delivered into cell membranes. Healthy and malignant colorectal tissues were used as the target cells. Cell suspensions of living tissues were studied in physiological conditions. For the maintenance of the surgically removed tissues, McCoy’s 5A culture medium was used. 5-DSA probe was rapidly delivered from perfluoropolyether (PFPE)/betaine mixed vesicles to the membranes of both healthy and malignant colorectal cells. The analysis of the computed 5-DSA ESR line shapes ensured that no spectral differences occurred when 5-DSA was either introduced directly into the cells or through the intervention of vesicles. This well agreed for an unmodified physical status of the membranes where the probe was mainly localized. |
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Keywords: | Betaine Cancer therapy Carrier systems ESR Mixed fluorinated vesicles |
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