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Preparation and characterization of magnetic levan particles as matrix for trypsin immobilization |
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| Authors: | JC MacielPL Andrad DFM Neri LB Carvalho JrCA Cardoso GMT CalazansJ Albino Aguiar MPC Silva |
| Institution: | a Programa de Pós-Graduação em Ciências Biológicas, Universidade Federal de Pernambuco, Cidade Universitária, 50670-901 Recife, PE, Brazil b Programa de Pós-Graduação em Ciência de Materiais, Universidade Federal de Pernambuco, Cidade Universitária, 50679-901 Recife, PE, Brazil c Universidade Federal do Vale do São Francisco, 56304-205 Petrolina, PE, Brazil d Departamento de Bioquímica, Universidade Federal de Pernambuco, Cidade Universitária, 50679-901 Recife, PE, Brazil e Departamento de Física, Universidade Federal de São Carlos, 13565-905 São Carlos, PE, Brazil f Departamento de Antibióticos, Universidade Federal de Pernambuco, Cidade Universitária, 50670-901 Recife, PE, Brazil g Departamento de Física, Universidade Federal de Pernambuco, Cidade Universitária, 50679-901 Recife, PE, Brazil |
| Abstract: | Magnetic levan was synthesized by co-precipitating D-fructofuranosyl homopolysaccharide with a solution containing Fe2+ and Fe3+ in alkaline conditions at 100 °C. The magnetic levan particles were characterized by scanning electron microscopy (SEM), magnetization measurements, X-ray diffractometry (XRD) and infrared spectroscopy (IR). Afterwards, magnetic levan particles were functionalized by NaIO4 oxidation and used as matrices for trypsin covalent immobilization. Magnetite and magnetic levan particles were both heterogeneous in shape and levan-magnetite presented bigger sizes compared to magnetite according to SEM images. Magnetic levan particles exhibited a magnetization 10 times lower as compared to magnetite ones, probably, due to the coating layer. XRD diffractogram showed that magnetite is the dominant phase in the magnetic levan. Infrared spectroscopy showed characteristics absorption bands of levan and magnetite (O-H, C-O-C and Fe-O bonds). The immobilized trypsin derivative was reused 10 times and lost 16% of its initial specific activity only. Therefore, these magnetic levan particles can be proposed as an alternative matrices for enzyme immobilization. |
| Keywords: | Levan Magnetic particle Trypsin immobilization |
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