| Effects of high-energy-pulse-electron beam radiation on biomacromolecules |
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| 作者姓名： | ZHU Hong XU JingZao LI ShiQiang SUN XiaoYu YAO SiDe WANG ShiLong School of Life Science and Technology TongJi University Shanghai China Shanghai Institute of Applied Physics Chinese Academy of Sciences Shanghai China |
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| 作者单位： | ZHU Hong1，XU JingZao1，LI ShiQiang1，SUN XiaoYu1，YAO SiDe1，2 WANG ShiLong1 1 School of Life Science and Technology，TongJi University，Shanghai 200092，China; 2 Shanghai Institute of Applied Physics，Chinese Academy of Sciences，Shanghai 201800，China |
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| 基金项目： | Supported by the National Natural Science Foundation of China (Grant Nos. 30570376 and 50673078)，the Shanghai Key Fundamental Project (Grant No. 06JC14068)，the Innovation Program of Shanghai Municipal Education Commis-sion (Grant No. 08ZZ21) |
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| 摘 要： | To study the molecular mechanism of high mutation frequency induced by high-energy-pulse-electron (HEPE) beam radiation, the effects of HEPE radiation on yeast cells, plasma membrane, plasmid DNA, and protein activity were investigated by means of cell counting, gel electrophoresis, AO/EB double fluorescent staining, etc. The results showed that the viability of yeast cells declined statistically with increase of absorbed doses. The half lethal dose (LD50) was 134 Gy. HEPE beam radiation had little influence on the function of plasma membrane and protein, while it could induce much DNA damage of single strand breaks (SSB) and double strand breaks (DSB) that were required for gene mutation. The G-value for DSB formation of HEPE beam radiation in aqueous solution was 5.7 times higher than that caused by 60Co gamma rays. HEPE can be a new effective method for induced mutation breeding and deserves further research in the future.
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| 收稿时间： | 1 July 2007 |
| 修稿时间： | 24 August 2007 |
Effects of high-energy-pulse-electron beam radiation on biomacromolecules |
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| ZHU Hong,XU JingZao,LI ShiQiang,SUN XiaoYu,YAO SiDe, WANG ShiLong School of Life Science and Technology,TongJi University,Shanghai ,China, Shanghai Institute of Applied Physics,Chinese Academy of Sciences,Shanghai ,China.Effects of high-energy-pulse-electron beam radiation on biomacromolecules[J].Science in China(Chemistry),2008,51(1):86-91. |
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| Authors: | Zhu Hong Xu JingZao Li ShiQiang Sun XiaoYu Yao SiDe and Wang ShiLong |
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| Institution: | (1) School of Life Science and Technology, TongJi University, Shanghai, 200092, China;(2) Shanghai Institute of Applied Physics, Chinese Academy of Sciences, Shanghai, 201800, China |
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| Abstract: | To study the molecular mechanism of high mutation frequency induced by high-energy-pulse-electron (HEPE) beam radiation, the effects of HEPE radiation on yeast cells, plasma membrane, plasmid DNA, and protein activity were investigated by means of cell counting, gel electrophoresis, AO/EB double fluorescent staining, etc. The results showed that the viability of yeast cells declined statistically with increase of absorbed doses. The half lethal dose (LD50) was 134 Gy. HEPE beam radiation had little influence on the function of plasma membrane and protein, while it could induce much DNA damage of single strand breaks (SSB) and double strand breaks (DSB) that were required for gene mutation. The G-value for DSB formation of HEPE beam radiation in aqueous solution was 5.7 times higher than that caused by 60Co gamma rays. HEPE can be a new effective method for induced mutation breeding and deserves further research in the future. |
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| Keywords: | high-energy-pulse-electron beam biomacromolecule radiation damage |
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