Comparative study on determination of fumagillin in fish by normal and reversed phase chromatography |
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Authors: | J Fekete Zs Romvári I Gebefügi A Kettrup |
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Institution: | 1. Department of General and Analytical Chemistry, Technical University of Budapest, 1521, Budapest, Hungary 2. Institute of Oecological Chemistry, GSF, Ingolst?tter Landstra?e 1, 85764, Oberschlei?heim, Germany
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Abstract: | Summary Fumagillin is an antibiotic agent mostly used in the veterinary profession. A normal-phase liquid chromatographic method was
developed for the determination of this drug in fish matrices. A hexane-dichloromethanedioxan-2-PrOH-acetic acid (43:43:9:5:0.1
v/v %) eluent was used on a Perkin Elmer silica gel column. For validation of the process a reversed-phase HPLC method was
also developed (eluent: acetonitrile-water-bicyclohyxylamine 70:30:0.05 v/v %, with Spherisorb ODS, as stationary phase).
Recovery is about 100% and the limit of detection is 5 ng g−1 for meat samples.
Presented at: Balaton Symposium on High-Performance Separation Methods, Siófok, Hungary, September 3–5, 1997. |
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Keywords: | Column liquid chromatography Normal- and reversed-phase LC Fumagillin in fish samples |
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