Polarographic determination of cyanide as nickelcyano complex in blood plasma after selective extraction in a methylene blue impregnated polyethylene column |
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Authors: | D Bohrer P C do Nascimento S Garcia Pomblum E Seibert and L Machado de Carvalho |
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Institution: | (1) Department of Chemistry, Federal University of Santa Maria, BR-97111-970 Santa Maria, RS Brazil, BR |
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Abstract: | A method for the determination of cyanide in blood plasma by differential pulse polarography (DPP) is described without a
drastic acidification of the sample. Cyanide was determined as tetracyanonickelate(II)-anion complex after a microwave-acid
assisted cleanup and a selective complex extraction in a polyethylene methylene blue (PE-MB) impregnated column. The cyano
complex was eluted from the column with water/acetonitrile and determined by pulse-polarography at –380 mV (Ag/AgCl). The
linear range of calibration was obtained from 1.2 to 9.6 μg of cyanide with r = 0.99 and RSD = 9% of 1.2 μg of cyanide. A
detection limit of 40 μg L–1 was calculated and the recoveries of cyanide from spiked samples were about 80%. This method was compared with the classical
pyridine-pyrazolone method.
Received: 3 September 1997 / Revised: 21 January 1998 / Accepted: 24 January 1998 |
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