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Separation, purification and structure elucidation of a polysaccharide from root of Cudrania tricuspidata
Authors:Lei Shi  Kaoshan Chen  Qun Dong  Jinian Fang  Kan Ding
Institution:(1) School of Life Science, Shandong University, Jinan, 250100, China;(2) Shanghai Institute of Materia Medica, Chinese Academy of Sciences, Shanghai, 201203, China
Abstract:A water-soluble polysaccharide (CPS-0) was obtained from the root of Cudrania tricuspidata (Carr.) Bur., by hot water extraction (70°C), deproteination using enzymolysis and Sevag method, precipitation with ethanol, and fractionation through DEAE-Sephadex A-50 chromatography. The purity of CPS-0 was determined by HPLC and the structure was elucidated by monosaccharide composition analysis, methylation analysis, GC, GC-MS, NMR spectral (1H-NMR, 13C-NMR, HMQC), UV, IR, and elemental analysis. The CPS-0 was found to contain glucose residues only. The average repeating unit is a decasaccharide having a backbone consisting of 1,4-linked α-D-glucopyranosyl residues to which the side chain consisting of terminal and 1,4-linked α-D-glucopyranosyl residues was attached at position 6 of the branching residues. __________ Translated from Chemical Journal of Chinese Universities, 2007, 28(6): 1088–1091 译自: 高等学校化学学报]
Keywords:root of Cudrania tricuspidata (Carr  ) Bur    polysaccharide  separation and purification  structural characterization
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