| Effects of Substrate Hydrophobicity/Hydrophilicity on Height Measurement of Individual DNA Molecules |
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| 作者姓名： | 王化斌 周星飞 安红杰 郭云昌 孙洁林 张益 胡钧 |
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| 作者单位： | [1]Shanghai Institute of Applied Physics, Chinese Academy of Sciences, Shanghai 201800 [2]Department of Physics, Ningbo University, Ningbo 315211 [3]Tianjin University of Science and Technology, Tianjin 300040 [4]Bio-X Life Science Research Center, Shanghai Jiao Tong University, Shanghai 200030 [5]Graduate School of the Chinese Academy of Sciences, Beijing 100080 |
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| 摘 要： | Effects of substrate hydrophobicity/hydrophilicity on height measurement of individual ds-DNA molecules are investigated with tapping mode atomic force microscopy （TMAFM） and vibrating mode scanning polarization force microscopy （VSPFM）. The measured heights of ds-DNA on hydrophobic highly oriented pyrolytic graphite （HOPG） are remarkably less than those on hydrophilic bare mica and Ni^2＋ treated mica in both TMAFM and VSPFM. By analysing the results, we propose that the hydrophobicity/hydrophilicity of substrate can greatly influence the height measurement of DNA molecules.
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| 关 键 词： | 疏水性 亲水性 个体 DNA分子 |
| 收稿时间： | 2006-10-30 |
| 修稿时间： | 2006-10-30 |
Effects of Substrate Hydrophobicity/Hydrophilicity on Height Measurement of Individual DNA Molecules |
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| WANG Hua-Bin,ZHOU Xing-Fei,AN Hong-Jie GUO Yun-Chang,SUNJie-Lin,ZHANG Yi,HU Jun.Effects of Substrate Hydrophobicity/Hydrophilicity on Height Measurement of Individual DNA Molecules[J].Chinese Physics Letters,2007,24(3):644-647. |
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| Authors: | WANG Hua-Bin ZHOU Xing-Fei AN Hong-Jie GUO Yun-Chang SUNJie-Lin ZHANG Yi HU Jun |
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| Institution: | 1.Shanghai Institute of Applied Physics, Chinese Academy of Sciences, Shanghai 201800 ; 2Department of Physics, Ningbo University, Ningbo 315211 ; 3 Tianjin University of Science and Technology, Tianjin 300040 ; 4 Bio-X Life Science Research Center, Shanghai Jiao Tong University, Shanghai 200030 ; 5 Graduate School of the Chinese Academy of Sciences, Beijing 100080 |
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| Abstract: | Effects of substrate hydrophobicity/hydrophilicity on height measurementof individual ds-DNA molecules are investigated with tapping mode atomicforce microscopy (TMAFM) and vibrating mode scanning polarization forcemicroscopy (VSPFM). The measured heights of ds-DNA on hydrophobic highlyoriented pyrolytic graphite (HOPG) are remarkably less than those onhydrophilic bare mica and Ni 2+ treated mica in both TMAFM and VSPFM. By analysing the results, we propose that the hydrophobicity/hydrophilicity of substrate can greatly influence the height measurement of DNA molecules. |
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| Keywords: | 06 60 Ei 07 79 Lh |
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