Mechanical properties of single motor molecules studied by three-dimensional thermal force probing in optical tweezers. |
| |
Authors: | Sylvia Jeney Ernst H K Stelzer Helmut Grubmüller Ernst-Ludwig Florin |
| |
Affiliation: | Institut de Physique de la Matière Complexe Faculté des Sciences de Base, Ecole Polytechnique Fédérale de Lausanne 1015 Lausanne, Switzerland. |
| |
Abstract: | A new method combining three-dimensional (3D) force measurements in an optical trap with the analysis of thermally induced (Brownian) position fluctuations of a trapped probe was used to investigate the mechanical properties of a single molecule, the molecular motor kinesin. One kinesin molecule attached to the probe was bound in a rigorlike state to one microtubule. The optical trap was kept weak to measure the thermal forces acting on the probe, which were mainly counterbalanced by the kinesin tether. The stiffness of kinesin during stretching and compression with respect to its backbone axis were measured. Our results indicate that a section of kinesin close to the motor domain is the dominating element in the flexibility of the motor structure. The experiments demonstrate the power of 3D thermal fluctuation analysis to characterize mechanical properties of individual motor proteins and indicate its usefulness to study single molecule in general |
| |
Keywords: | mechanical properties molecular motors optical tweezers single‐molecule studies thermal fluctuations |
|