| 用离子交换法从发酵液中提取1,6-二磷酸果糖 |
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| 引用本文： | 邹霞娟,陈长坡,杨军,胡劲松,侯有佐. 用离子交换法从发酵液中提取1,6-二磷酸果糖[J]. 离子交换与吸附, 2000, 16(6): 528-533 |
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| 作者姓名： | 邹霞娟 陈长坡 杨军 胡劲松 侯有佐 |
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| 作者单位： | 北京市发酵工业研究所,北京 100022 |
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| 摘 要： | 在发酵生产１，６－二磷酸果糖的提取工艺中，对提取总率影响较大的离子交换工艺进行了研究，结果表明，用南开大学化工厂生产的ＮＫ－Ｄ３０１Ｃ１型树脂对已预处理的ＰＨ为２的发酵液中１，６－二磷酸果糖进行动态交换吸附，当上柱流速控制在２５ｍｌ／ｍｉｎ条件下，其交换量为０．７０６ｍｍｏｌ／ｍｌ，用０．０１ｍｏｌ／Ｌ ＮａＣｌ－０．０１５ｍｏｌ／Ｌ ＨＣｌ和０．４ｍｏｌ／ＬＮａＣｌ－０．１５ｍｏｌ／Ｌ ＨＣｌ分步洗脱，流速控制在２５ｍｌ／ｍｌ时，其洗涤效果最好，离子交换工序收率大于９０％。
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| 关 键 词： | 二磷酸果糖 树脂 离子交换 提取工艺 发酵 |
| 文章编号： | 1001-5493(2000)06-0528-06 |
| 修稿时间： | 1999-12-03 |
EXTRACTION OF FRUCTOSE 1,6-DIPHOSPHATE FROM FERMENTATION BROTH BY LON-EXCHANGE PROCESS |
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| ZOU Xiajuan CHEN changpo YANG Jun HU Jinsong HOU Youzuo Beijing Fermentation Industry Institute,Beijing. EXTRACTION OF FRUCTOSE 1,6-DIPHOSPHATE FROM FERMENTATION BROTH BY LON-EXCHANGE PROCESS[J]. Ion Exchange and Adsorption, 2000, 16(6): 528-533 |
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| Authors: | ZOU Xiajuan CHEN changpo YANG Jun HU Jinsong HOU Youzuo Beijing Fermentation Industry Institute Beijing |
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| Affiliation: | ZOU Xiajuan CHEN changpo YANG Jun HU Jinsong HOU Youzuo Beijing Fermentation Industry Institute,Beijing 100022 |
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| Abstract: | The study shows that fructose 1,6-diphosphate absorbing capacity of NK-D301 Cl resin was 0.706 mmol/ml of wet resin and the efficiency of the elusion was higher by 0.01mol/L NaCl-0.015mol/L HCl and 0.4mol/L NaCl-0.015mol/L HCl as elusion at the flow rate of 1/200vvm. Under the controlled condition, the extraction yield of fructose 1,6-diphosphate reached over 90%. |
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| Keywords: | Fructose 1 6-Diphosphate resin Ion-exchange process. |
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