Development of a tandem thin-layer chromatography-high-performance liquid chromatography method for the identification and determination of corticosteroids in cosmetic products |
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Authors: | Luigi GagliardiDaniela De Orsi,Maria Rosaria Del GiudiceFranco Gatta,Rita Porrà Paola Chimenti,Domenica Tonelli |
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Affiliation: | a Laboratorio di Chimica del Farmaco, Istituto Superiore di Sanità, Rome, Italy b Dipartimento di Studi di Chimica e Tecnologia delle Sostanze Biologicamente Attive, Università La Sapienza, Rome, Italy c Dipartimento di Chimica Fisica ed Inorganica, Università di Bologna, Viale del Risorgimento 4, 40136 Bologna, Italy |
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Abstract: | A solid-phase extraction clean-up and and a liquid chromatographic method with ultraviolet detection were developed for the analysis of 51 corticosteroids in cosmetic samples in order to screen commercial samples for the presence of undeclared synthetic corticosteroids. A thin-layer chromatographic analysis was carried out on silica gel plates, using different eluants and detection reagents. When such a preliminary chromatographic separation gave some indications about the presence of steroid compounds, the methanol extracts from real samples were applied to a solid-phase extraction C18 cartridge, and the analytes eluted with ethyl ether. The high-performance liquid chromatographic separation was then carried out for the identification and determination of the analytes using a Purospher RP-18 column, an isocratic or a gradient elution with a mixture acetonitrile-water and a photodiode-array detector. The accuracy of the method was determined by spiking experiments on home-made cosmetic samples. The analytical recoveries were satisfactory. |
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Keywords: | Corticosteroids Cosmetic products analysis HPLC TLC SPE |
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