Separation and determination of homovanillic acid and vanillylmandelic acid by capillary electrophoresis with electrochemical detection |
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Authors: | Xiujun LiWenrui Jin Qianfeng Weng |
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Institution: | School of Chemistry and Chemical Engineering, State Key Laboratory of Microbial Technology, Shandong University, Jinan 250100, China |
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Abstract: | A method of separation and determination of homovanillic acid (HVA) and vanillylmandelic acid (VMA) was developed based on capillary zone electrophoresis/amperometric detection with high sensitivity, good resolution and selectivity. In order to achieve complete separation and good response, several factors including pH, buffer concentration, separation voltage, detection potential and the length of separation capillary, were studied in detail. The method has been used to determine both HVA and VMA in human urine. Uric acid (UA) in human urine did not interference with their determination. The limit of detection of the method was 1.3×10−6 mol/l (1.4 fmol) for HVA and 7.9×10−7 mol/l (0.87 fmol) for VMA at a signal-to-noise ratio of 3. |
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Keywords: | Capillary electrophoresis Electrochemical detection Homovanillic acid Vanillylmandelic acid Urine |
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