Simple and convenient chemiluminescence method for the determination of melatonin |
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Authors: | Jianzhong LuChoiwan Lau Myung Koo Lee Masaaki Kai |
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Affiliation: | a School of Pharmaceutical Sciences, Nagasaki University, 1-14 Bunkyo-Machi, Nagasaki 852-8521, Japan b College of Pharmacy, Chungbuk National University, San 48, Kaoshin-Dong, Heungduk-ku, Cheongju 361-763, South Korea |
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Abstract: | A simple and convenient chemiluminescence (CL) method for the determination of melatonin is reported. It is based on the observation that melatonin can greatly enhance the ultra-weak CL between H2O2 and acetonitrile in alkaline solution. Light emission is intense and long-lived, and even with a simple setup, a high sensitivity could be achieved. The log-log linear range was 250 nM-250 μM with a 3σ detection limit of 100 nM. Singlet oxygen is suggested to be produced by the reaction between H2O2 and acetonitrile and is responsible for the CL of melatonin. As a preliminary application, this simple method has been successfully applied into the determination of melatonin in health-foods. |
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Keywords: | Melatonin Chemiluminescence Acetonitrile Singlet oxygen |
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