Fast determination of flavonoids in Glycyrrhizae radix by capillary zone electrophoresis |
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Authors: | Tao BoKe An Li Huwei Liu |
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Institution: | The College of Chemistry and Molecular Engineering, Peking University, Beijing 100871, China |
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Abstract: | A fast capillary zone electrophoresis (CZE) method has been developed for the determination of four flavonoids (liquiritin, licoisoflavone A, licochalconel A and calycosin) in Glycyrrhizae radix. After a series of optimization experiments, 100 mM borate buffer (pH 10.5), 30 kV applied voltage and 35 °C temperature were selected. The contents of four flavonoids in cultivated and wild crude drugs of Glycyrrhizae radix with different growth periods from one to four years, collected from different areas were successfully determined within 8 min, with satisfactory repeatability and recovery. |
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Keywords: | Capillary zone electrophoresis Fast determination Flavonoids Glycyrrhizae radix |
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