Screen-printed biosensors for the control of wine quality based on lactate and acetaldehyde determination |
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Authors: | Alina Avramescu Thierry NoguerMaria Avramescu Jean-Louis Marty |
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Affiliation: | a Faculty of Chemistry, University of Bucharest, 90-92 Sos. Panduri, Bucharest, Romania b Centre de Phytopharmacie, Université de Perpignan, UMR CNRS 5054, 52 avenue de Villeneuve, 66860 Perpignan Cedex, France c Research Institute for Viticulture and Enology, Valea Calugareasca, 2040, Prahova, Romania |
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Abstract: | Biosensors for d-lactate and acetaldehyde were developed, based on screen-printed electrodes and NAD+-dependent dehydrogenases. Modification of screen-printed electrodes with the mediator Meldola Blue or with Meldola Blue-Reinecke salt resulted in sensitive, low cost and reliable NADH detectors. The biosensors were realised in two configurations, as disposable and reusable devices. Single-use sensors were obtained by simple deposition of enzyme and cofactor on the surface of mediator-modified electrodes. Chronoamperometry was used for the detection of substrates in small volumes of samples (25 μl). Immobilisation of dehydrogenases by entrapment in poly(vinyl alcohol) bearing styrylpyridinium groups (PVA-SbQ) allowed sensors to be obtained with sufficient operational stability. Amperometry in stirred solutions was the detection technique with biosensors for multiple use. The 3σ detection limits for acetaldehyde were 1 μM by amperometry and 6 μM by chronoamperometry and for d-lactate-0.03 μM and 0.05 μM for reusable and disposable biosensors respectively. The biosensors were applied in the analysis of some French and Romanian wines. |
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Keywords: | NADH Meldola Blue Screen-printed electrodes smallcaps" >d-Lactate Acetaldehyde Wine |
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