| Abstract: | The electrochemical behavior of a glassy carbon paste electrode (GCPE) is evaluated in comparison to that of graphite paste electrode (gPE) and glassy carbon electrode (GCE). Important shifting in the peak potentials and increases in the peak currents for catechol, ascorbic acid, dopamine and hydroquinone were obtained for the GCPE and its usefulness for the development of phenol and catechol biosensors was also evaluated. Both, pure mushroom polyphenol oxidase (PPO) and fresh mushroom tissues were used as biorecognition elements. The effect of the binder percentage in the composite material was also studied. The bioelectrode was used for the determination of dopamine and acetaminophen in pharmaceutical formulations and for the detection of polyphenols in wine and tea. The bioelectrode demonstrated to be very stable as the response remained around 90% after four months at 4 °C. |
