Determination of puerarin and daidzein in Puerariae radix and its medicinal preparations by micellar electrokinetic capillary chromatography with electrochemical detection |
| |
Authors: | Yuhua CaoChanggang Lou Xin ZhangQingcui Chu Yuzhi FangJiannong Ye |
| |
Affiliation: | Department of Chemistry, East China Normal University, ZhongShaan Road North 3663, Shanghai 200062, China |
| |
Abstract: | The use of micellar electrokinetic capillary chromatography (MECC) with electrochemical detection is described for the determination of puerarin and daidzein in Puerariae radix and its medicinal preparations. Operated in a wall-jet configuration, a 300 μm diameter carbon-disk electrode was used as the working electrode, which exhibits good responses at +900 mV (versus SCE) for the two analytes. Under the optimum conditions, the analytes were base-line separated within 11 min in a sodium dodecyl sulphate—borax (pH 7.8) running buffer, and excellent linearity was obtained in the concentration range from 5.0×10−4 to 5.0×10−6 mol/l. The detection limit (S/N=3) was 6×10−7 and 1.1×10−6 mol/l for puerarin and daidzein, respectively. This work provides a useful method for the analysis of traditional Chinese medicines. |
| |
Keywords: | Puerariae radix Puerarin Daidzein Micellar electrokinetic capillary chromatography Electrochemical detection |
本文献已被 ScienceDirect 等数据库收录! |