Heterogeneous and homogeneous electrochemiluminoimmunoassays of hTSH at disposable oxide-covered aluminum electrodes |
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Authors: | S Kulmala M HåkanssonA-M Spehar A NymanJ Kankare K LoikasT Ala-Kleme J Eskola |
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Institution: | a Laboratory of Inorganic and Analytical Chemistry, Helsinki University of Technology. P.O. Box 6100, FIN-02015 HUT, Finland b Laboratory of Analytical Chemistry, University of Turku, FIN-20014 Turku, Finland c Labmaster Oy, P.O. Box 71, FIN-20521 Turku, Finland |
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Abstract: | Heterogeneous and homogeneous immunoassays of human thyroid stimulating hormone (hTSH) were developed on immunometric basis using aromatic Tb(III) chelates as electrochemiluminescent labels and varied types of disposable oxide-covered aluminum electrodes as the solid phase of the immunoassays. The long luminescence lifetime of the present labels allows the use of time-resolved electrochemiluminescence detection and provide the low detection limits of these labels and, thus, sensitive immunoassays. The primary antibody of immunometric immunoassays was coated upon aluminum oxide surface by physical absorption. In homogeneous immunoassays using 66 μl cell and 15 min incubation time, a linear calibration range of 0.25-324 μU/ml was obtained by applying only a single cathodic excitation pulse in the detection step of the assay. |
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Keywords: | Electrochemiluminescence Electrogenerated chemiluminescence Hot electrons Thin insulating-film coated electrodes Immunoassay Bioaffinity assay Tb(III) chelates |
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