| 固定化肌红蛋白在水-乙醇混合溶液中的类酶活性电化学 |
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| 引用本文： | 李业梅,刘慧宏. 固定化肌红蛋白在水-乙醇混合溶液中的类酶活性电化学[J]. 分析试验室, 2012, 0(4): 64-68 |
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| 作者姓名： | 李业梅 刘慧宏 |
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| 作者单位： | 郧阳师范高等专科学校化学系;武汉纺织大学化学与化工学院 |
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| 基金项目： | 湖北省教育厅重点科研项目(D200960004)资助 |
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| 摘 要： | 用琼脂糖将肌红蛋白(Mb)固定到玻碳电极(GC)表面,制备了Mb-琼脂糖膜修饰电极。包埋在琼脂糖膜中的Mb在缓冲溶液和乙醇混合溶液中与电极直接传递电子,得到一对对称的Mb辅基血红素Fe(III)/Fe(II)电对的可逆氧化还原峰。其式电势随缓冲溶液pH值增加而负移,且呈线性关系,这说明Mb的电子传递过程伴随有质子的转移。在缓冲溶液和乙醇混合溶液中,固定化肌红蛋白表现出类似细胞色素P450的催化活性,能快速催化还原氯乙烷(六氯乙烷、五氯乙烷、四氯乙烷)脱氯,Mb-琼脂糖膜修饰电极具有较好的稳定性和重现性,可用于这些物质的定量检测。
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| 关 键 词： | 肌红蛋白 琼脂糖 乙醇-水混合溶液 电化学 电催化 |
Electrochemical study on enzyme-like activities of immobilized myoglobin in aqueous-ethanol solvent mixtures |
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| LI Ye-mei,and LIU Hui-hong. Electrochemical study on enzyme-like activities of immobilized myoglobin in aqueous-ethanol solvent mixtures[J]. Chinese Journal of Analysis Laboratory, 2012, 0(4): 64-68 |
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| Authors: | LI Ye-mei and LIU Hui-hong |
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| Affiliation: | 1.Department of Chemistry,Yunyang Teachers College,Danjiangkou 442700;2.College of Chemistry and Chemical Engineering,Wuhan Textile University,Wuhan 430074) |
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| Abstract: | Myoglobin was immobilized on the surface of a glassy carbon electrode(GCE) by agarose hydrogel.Myoglobin entrapped in the agarose film undergoes fast direct electron-transfer reaction.The formal potential(E0′) was linearly dependent on pH value of the solution,indicating that the electron transfer is proton-coupled.The immobilized Mb is able to dehalogenate chloroethanes(hexachloroethane,pentachloroethane,tetrachloroethane) which is cytochrome P450-like activity.Stability and reproducibility of the modified electrode make it possible to be used for the determination of these substances. |
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| Keywords: | Myoglobin Agarose Aqueous-ethanol solvent mixtures Electrochemistry Electrocatalysis |
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