Electrochemical quantification of reactive oxygen and nitrogen: challenges and opportunities |
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| Authors: | Sabine Borgmann |
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| Institution: | 1.Biologisch-Chemische Mikrostrukturtechnik, Fakult?t Chemie,Technische Universit?t Dortmund,Dortmund,Germany;2.ISAS—Institute for Analytical Science,Dortmund,Germany |
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| Abstract: | Reactive oxygen species (ROS) and reactive nitrogen species (RNS) play a crucial role in chemical signaling processes of biological
cells. Electrochemistry is one of the rare methods able to directly detect these species. ROS and RNS can be monitored in
the local microenvironment of cells in real time at the site where the actual signaling takes place. This review presents
recent advances made with amperometric electrochemical techniques. Existing challenges for the quantification of ROS and RNS
in biological systems are discussed to promote the development of innovative and reliable cell-based assays.
Figure Reactive oxygen and nitrogen species (ROS & RNS) are produced biological cells. An amperometric sensor is placed in close
proximity. The recorded current I is used to determine fluxes of certain species.
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| Keywords: | Cell systems/single cell analysis Electrochemical sensors Bioanalytical methods Reactive oxygen species Reactive nitrogen species Cell signaling |
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