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PREFERENTIAL INHIBITION OF NUCLEOSOME ASSEMBLY BY ULTRAVIOLET-INDUCED (6-4)PHOTOPRODUCTS |
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| Authors: | Hideki Matsumoto Akira Takakusu Toshio Mori Makoto Ihara Takeshi Todo Takeo Ohnishi |
| Affiliation: | Department of Anatomy, Nara Medical University, 840 Shijo-cho, Kashihara, Nara 634, Japan;Radioisotope Center, Nara Medical University, 840 Shijo-cho, Kashihara, Nara 634, Japan;Department of Biology, Nara Medical University, 840 Shijo-cho, Kashihara, Nara 634, Japan;Department of Radiation Biology, Faculty of Medicine, Osaka University, Yamada-oka2–2, Suita, Osaka 565, Japan |
| Abstract: | We reconstituted nucleosomes in vitro using two kinds of damaged pBR322 plasmid DNA carrying cyclobutane pyrimidine dimers (CPD) or (6-4)photoproducts. The results indicate that nucleosome assembly is inhibited preferentially by (6-4)photoproducts compared with CPD, suggesting that the regions carrying (6-4)photoproducts retain their nucleosome-free form, i.e. linker-like conformation until completion of the repair processes. |
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