Quantitative intact specimen magnetic resonance microscopy at 3.0 T |
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Authors: | Kevin G. Bath Henning U. Voss Deqiang Jing Stewart Anderson Barbara Hempstead Francis S. Lee Jonathan P. Dyke Douglas J. Ballon |
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Affiliation: | 1. Department of Psychiatry, Weill Cornell Medical College of Cornell University, 1300 York Avenue, New York, NY 10065, USA;2. Department of Radiology, Weill Cornell Medical College of Cornell University, 1300 York Avenue, New York, NY 10065, USA;3. Department of Physiology, Biophysics, and Systems Biology, Weill Graduate School of Medical Sciences, Cornell University, 1300 York Avenue, New York, NY 10065, USA;4. Division of Hematology and Medical Oncology, Department of Medicine, Weill Cornell Medical College of Cornell University, 1300 York Avenue, New York, NY 10065, USA |
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Abstract: | In this report, we discuss the application of a methodology for high-contrast, high-resolution magnetic resonance microscopy (MRM) of murine tissue using a 3.0-T imaging system. We employed a threefold strategy that included customized specimen preparation to maximize image contrast, three-dimensional data acquisition to minimize scan time and custom radiofrequency resonator design to maximize signal sensitivity. Images had a resolution of 100×78×78 μm3 with a signal-to-noise ratio per voxel greater than 25:1 and excellent contrast-to-noise ratios over a 30-min acquisition. We quantitatively validated the methods through comparisons of neuroanatomy across two lines of genetically engineered mice. Specifically, we were able to detect volumetric differences of as little as 9% between genetically engineered mouse strains in multiple brain regions that were predictive of underlying impairments in brain development. The overall methodology was straightforward to implement and provides ready access to basic MRM at field strengths that are widely available in both the laboratory and the clinic. |
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Keywords: | Magnetic resonance microscopy (MRM) Mouse Neuroanatomy TrkB FoxG1 |
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