| 活化高分子膜免疫电极检测乳腺癌抗原 |
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| 引用本文： | 彭图治,祝方猛,程琼.活化高分子膜免疫电极检测乳腺癌抗原[J].分析化学,2001,49(4):383-386. |
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| 作者姓名： | 彭图治 祝方猛 程琼 |
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| 作者单位： | 浙江大学西溪校区化学系, |
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| 基金项目： | 中国科学院长春应用化学研究所电分析化学开放研究实验室资助，浙江省自然科学基金资助项目 |
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| 摘 要： | 报道了一种灵敏度高和使用简便的免疫电极，用于测定乳腺癌抗原（CA15－3）。CA15－3抗体固定在用羟胺活化的聚氯乙烯（PVC）－牛血清白蛋白（BSA）基体膜上。当抗体膜与CA15－3血清结合形成抗原体复合物膜后，将该复合物膜固定在自制Ag-AgCI基体电极顶端，利用毫伏计测定膜电位。该法可在15－240U／mL范围测定CA15－3，线性关系良好，回归方程：△E=-8、84 13、97IgCA15-3]，相关系数0．9998。人血清中其他常见抗原对CA15－3测定基本无干扰。同时，探讨了免疫膜的电位响应机理。
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| 关 键 词： | 乳腺癌抗原 电位法 免疫传感器 活化高分子膜免疫电极 检测 聚氯乙烯 牛血清血蛋白 免疫分析 |
Determination of Cancer Antigen 15-3 Using an Activated Polymer Membrane Immunoelectrode |
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| Peng Tuzhi,Zhu Fangmeng,CHENG Qiong.Determination of Cancer Antigen 15-3 Using an Activated Polymer Membrane Immunoelectrode[J].Chinese Journal of Analytical Chemistry,2001,49(4):383-386. |
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| Authors: | Peng Tuzhi Zhu Fangmeng CHENG Qiong |
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| Abstract: | A potentiometric immunoelectrode with non-labeled anticancer antigen CA 15-3 antibody used for determinating CA15-3 was reported. firstly, the anti-CA15-3 antibody was immobilized on the surface of polyvinyl chloride-bovine serum albumin membrane, which was activated previously. Secondly, CA15-3 was added on the surface of the antibody membrane to form the antigen-antibody complex. Lastly, the complex membrane was used to assemble an immunoelectrode with an Ag-AgCl inner electrode. The potential of the complex membrane was measured with a pH meter. The response was linear over the range of 15～240 U／mL with a correlation coefficient of 0.9998. Other common antigens in human serum did not interfere with the determination of CA15-3. The mechanism of potential response was explored. |
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| Keywords: | Cancer antigen15-3 potentiometry immunoelectrode |
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