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Direct determination of iron in ferritin using the polarographic catalytic current |
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| Authors: | Jiang Qiu Junfeng Song Hong Gao |
| Institution: | (1) Institute of Electroanalytical Chemistry, Northwest University, Xian, 710069, People s Republic of China;(2) Present address: Research Institute of Biochemical Engineering, East China University of Science and Technology, 200237 Shanghai, Peoples Republic of China |
| Abstract: | A new method for the determination of iron in ferritin using the polarographic catalytic current has been developed. Iron is released in NaOH and triethanolamine (TEA) solution and subsequently determined based on the polarographic catalytic wave in the presence of bromate with the peak potential of –1.0 V (vs.SCE). The 2.5-derivative peak height of iron is linearly proportional to its concentration in the range of 8.0 × 10–8–1.0 × 10–6 g/ml with a correlation coefficient of 0.9991. The method has been applied to the determination of iron in human fetal liver and horse-spleen ferritin and the recovery is 95–112%. |
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