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A highly sensitive and stable detection of acetylcholine by HPLC-osmium-horseradish peroxidase redox polymer electrode coated on a gold radial flow ring disk |
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| Authors: | Katsunobu Yamamoto Toshiyuki Chikuma |
| Affiliation: | a Laboratory of Research and Development, Bioelectro Analytical Science Japan Inc., 1-36-6 Oshiage, Sumida-ku, Tokyo 131-0045, Japan b Laboratories of Molecular Recognition and Natural Information Science, Graduate School of Integrated Science, Yokohama City University, 22-2 Seto, Kanazawa-ku, Yokohama 236-0027, Japan c Department of Hygienic Chemistry, Showa Pharmaceutical University, Machida 194-8543, Japan |
| Abstract: | To detect a low level of acetylcholine (Ach) output in the dialysate of rat frontal cortex, osmium-peroxidase redox polymer modified gold-ring disk electrode (Os-HRP-RDE) was fabricated in high-performance liquid chromatography (HPLC)-electrochemical detection (ECD). In comparison with platinum electrode, the sensitivity of acetylcholine detection by Os-HRP-RDE was stable during a day, the coefficient of variation was 0.95%, and the decrease of the sensitivity for 1 week was 5.4%. The Os-HRP-RDE could detect lower than 3 fmol of acetylcholine. The present technique, in the absence of acetylcholinesterase (AchE) inhibitor, is useful to facilitate physiological investigations of cholinergic neuronal activity in the brain. |
| Keywords: | Acetylcholine HPLC Os-HRP Au-ring disk Microdialysis |
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