Determination of selenoamino acids by gas chromatography-mass spectrometry |
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Authors: | Beril Is?cio?lu,Emü r Henden |
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Affiliation: | a Laboratory of Instrumental Analysis, Regional Institute for Hygiene, 35350 Izmir, Turkey b Chemistry Department, Faculty of Science, Ege University, Bornova, 35100 Izmir, Turkey |
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Abstract: | The application of the recently introduced ethylchloroformate derivatization method for the separation and determination of selenomethionine and selenocystein in selenium-enriched yeast and yeast-free tablets by means of a gas chromatography-mass spectrometry (GC-MS) system has been studied. The efficiency of three methods for the extraction of selenomethionine from the tablets were compared. Total selenium content of the same tablets were measured using inductively coupled plasma (ICP)-MS and it was found that in the selenized yeast tablets about 80% of the total selenium is present as selenomethionine. The results were in agreement with the values in the labels and with the literature. The accuracy of the total selenium analysis was controlled by the analysis of a reference material. |
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Keywords: | Gas chromatography-mass spectrometry (GC-MS) Selenomethionine Selenocysteine Selenocystine Nutritional supplements Yeast |
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