New potentiometric microbial biosensor for ethanol determination in alcoholic beverages |
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| Authors: | Lucian Rotariu Camelia Bala Vasile Magearu |
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| Affiliation: | Laboratory of Quality Control and Process Monitoring, Department of Analytical Chemistry, Faculty of Chemistry, University of Bucharest, 90 Sos. Panduri, 050663 Bucharest 5, Romania |
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| Abstract: | A potentiometric oxygen electrode was successfully used to produce a microbial biosensors with an extended response range, based on immobilised yeast cells (Saccharomyces ellipsoideus). The response for ethanol takes ca. 7 min for the steady-state method and 2 min for kinetic measurements. A linear calibration achieved over more than two decades of ethanol concentration. Selective determination of ethanol in the presence of non-volatile compounds was achieved using a second PTFE membrane to cover the biocatalytic layer. |
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| Keywords: | Ethanol Microbial biosensor Potentiometry Food analysis |
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