Screen-printed enzyme electrodes for the detection of marker analytes during winemaking |
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| Authors: | A Lupu G Palleschi |
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| Institution: | a Dipartimento di Scienze e Tecnologie Chimiche, University of Rome “Tor Vergata”, via della Ricerca Scientifica 1, 00133 Rome, Italyb Dipartimento di Scienze degli Alimenti, University of Teramo, via Spagna 1, Mosciano S. Angelo, 64023 Teramo, Italy |
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| Abstract: | Biosensors for malic acid and glucose have been developed, using screen-printed electrodes and two different classes of enzymes: NAD(P)+-dependent dehydrogenases and oxidases. The active surface of the electrodes was modified using Meldola Blue (malic acid) and Prussian Blue (glucose) and in this way sensitive, low cost and reliable NAD(P)H and H2O2 probes were obtained. Fixed potential amperometry was used for the detection of substrates in small volumes of sample (50 μl). Immobilization of the enzymes in a polyethylenimine-glutaraldehyde cross-linking membrane allowed sensors to be obtained with sufficient operational stability. The detection limits were of 10−5 M for malic acid and 10−6 M for glucose. The sensors were applied in the analysis of different samples of wine. |
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| Keywords: | NAD(P)H H2O2 Meldola Blue Prussian Blue Screen-printed electrodes Malic acid Glucose Wine |
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