Application of unfold principal component analysis and parallel factor analysis to the exploratory analysis of olive oils by means of excitation-emission matrix fluorescence spectroscopy

Discrimination between virgin olive oils and pure olive oils is of primary importance for controlling adulterations. Here, we show the potential usefulness of two multiway methods, unfold principal component analysis (U-PCA) and parallel factor analysis (PARAFAC), for the exploratory analysis of the two types of oils. We applied both methods to the excitation-emission fluorescence matrices (EEM) of olive oils and then compared the results with the ones obtained by multivariate principal component analysis (PCA) based on a fluorescence spectrum recorded at only one excitation wavelength. For U-PCA and PARAFAC, the ranges studied were λ_ex=300-400 nm, λ_em=400-695 nm and λ_ex=300-400 nm, λ_em=400-600 nm. The first range contained chlorophylls, whose peak was much more intense than those of the rest of species. The second range did not contain the chlorophylls peak but only the fluorescence spectra of the remaining compounds (oxidation products and Vitamin E). The three-component PARAFAC model on the second range was found to be the most interpretable. With this model, we could distinguish well between the two groups of oils and we could find the underlying fluorescent spectra of three families of compounds.