Flow-injection chemiluminescence assay for ultra-trace determination of DNA using rhodamine B-Ce(IV)-DNA ternary system in sulfuric acid media |
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Authors: | Yongjun Ma |
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Affiliation: | Institute of Chemistry, Northwest Normal University, Lanzhou 730070, China |
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Abstract: | A novel flow-injection chemiluminescence method for the determination of DNA at ultra-trace level has been established. In 0.8 M sulfuric acid media, the chemiluminescence of the rhodamine B-cerium (IV) or Ce(IV) system is enhanced by DNA, activated previously by imidazole-HCl buffer solution (pH 7.0). The enhanced intensity of chemiluminescence is in proportion to log DNA concentration 1.0×10−8 to 0.1 μg ml−1 for herring sperm DNA and 2.0×10−6 to 0.2 μg ml−1 for calf thymus DNA with 3σ detection limits of 8.3×10−9 μg ml−1 for herring sperm DNA and 3.5×10−7 μg ml−1 for calf thymus DNA, respectively. The relative standard deviation for 1.0×10−4 μg ml−1 herring sperm DNA was 0.99% and 2.0×10−3 μg ml−1 for calf thymus DNA was 1.1% (n=11). Using the optimized system, DNA contents in six synthetic samples has been determined with recoveries of 99.5-109.0%. The possible mechanism has also been studied in this paper. |
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Keywords: | Flow-injection Chemiluminescence DNA Rhodamine B |
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