Droplet-based microfluidic lab-on-a-chip for glucose detection |
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Authors: | Vijay Srinivasan Vamsee K Pamula Richard B Fair |
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Affiliation: | Department of Electrical Engineering, Duke University, 130 Hudson Hall, Durham, NC 27708, USA |
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Abstract: | A microfluidic lab-on-a-chip (LoC) platform for in vitro measurement of glucose for clinical diagnostic applications is presented in this paper. The LoC uses a discrete droplet format in contrast to conventional continuous flow microfluidic systems. The droplets act as solution-phase reaction chambers and are manipulated using the electrowetting effect. Glucose is measured using a colorimetric enzyme-kinetic method based on Trinder’s reaction. The color change is detected using an absorbance measurement system consisting of a light emitting diode and a photodiode. The linear range of the assay is 9-100 mg/dl using a sample dilution factor of 2 and 15-300 mg/dl using a sample dilution factor of 3. The results obtained on the electrowetting system compare favorably with conventional measurements done on a spectrophotometer, indicating that there is no change in enzyme activity under electrowetting conditions. |
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Keywords: | Electrowetting Droplet Glucose Lab-on-a-chip Microfluidics |
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