Quick and sensitive HPLC separations on non-porous reversed-phase packings

Summary The aim of this work was to evaluate reversed-phase chemically bonded non-porous (micropellicular)d _p=1.5 μm stationary phases. On these modern phases the time for analysis of complex mixtures of solutes—whether monomeric or polymeric (e.g. drugs, vitamins, peptides, or protein)—is very short compared with that on porous phases. Different surface chemistries were elaborated for the separation of different types of sample. For the separation of small molecules a long-chain (C₁₄) hydrocarbon-coated phase seems to be optimum; a short chain (C₆) hydrocarbon bonded to the surface of the silica seems better for the separation of polymers. The efficiency, the low analysis times, and sensivities were demonstrated by separation of different proteins, peptides, drugs, alkaloids, and mixtures of water and fat-soluble vitamins.