飞秒激光与细胞的相互作用

近年来，高速发展的激光技术已经广泛应用于医疗领域，比如眼科手术和光学相干层析成像。尤为特殊的是，飞秒激光更可专用于对活体细胞的基础研究。飞秒激光具有别种激光无法获得的特殊性质。它对于细胞的伤害很小，因为它的线性吸收和热效应远远低于连续光。此外，由于飞秒光的超高峰值功率，它可以对细胞形成多光子电离而在细胞膜上开孔，因此可以转基因和融合细胞，同时不会太过于伤害细胞。在这个报告里，将阐述我们的以下研究：飞秒光致转基因、细胞融合以及细胞在凋亡中的动态过程。在单细胞水平上提出了一些凋亡过程的可能机制，如活性氧化合物、核管和细胞内自由钙离子的产生。

Experimental Interactions of Femtosecond Laser with Cells

Laser technologies have advanced rapidly in recent years and in fact have been applied in many applications such as eye surgery and optical coherence tomography in medicine.In particular,femtosecond(fs) lasers can also be used to study the fundamental processes within live cells.Femtosecond lasers possess unique features that are not achievable with other lasers. It induces minimal,if any,damage to the cell under study because it can generate much less linear absorption and heat than CW lasers.Besides,due to their high peak power,fs lasers can generate multi-photon ionization within cells and thus can perforate the cell membrane to enable transfection and cell fusion to take place,apparently without harming the cells.In this talk,Ⅰshall report our studies in transfection and cell fusion and the dynamics during cellular apoptosis introduced by fs beams.We propose probable mechanisms for the generation of reactive oxygen species,nuclear tubules and free calcium at the cellular level triggered by the fs beam.