A mussel tissue certified reference material for multiple phycotoxins. Part 3: homogeneity and stability |
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Authors: | McCarron Pearse Emteborg Håkan Giddings Sabrina D Wright Elliott Quilliam Michael A |
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Affiliation: | (1) National Research Council Canada, Institute for Marine Biosciences, 1411 Oxford St, B3H 3Z1 Halifax, NS, Canada;(2) European Commission, Joint Research Centre, Institute for Reference Materials and Measurements, Retieseweg 111, 2440 Geel, Belgium |
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Abstract: | A candidate certified reference material (CRM) for multiple shellfish toxins (domoic acid, okadaic acid and dinophysistoxins, pectenotoxins, yessotoxin, azaspiracids and spirolides) has been prepared as a freeze-dried powder from mussel tissues (Mytilus edulis). Along with the certified values, the most important characteristics for a reference material to be fit-for-purpose are homogeneity and stability. Acceptable between-bottle homogeneity was found for this CRM. Within-bottle homogeneity was assessed using domoic acid, and it was shown that repeated subsampling of the CRM can be performed precisely down to 0.35 g. Both short- and long-term stability studies carried out under isochronous conditions demonstrated excellent stability of the various toxins present in the material. While degradation of some analytes was observed at +60°C in short-term studies, it was determined that shipping at ambient temperature is adequate. No instability was detected in long-term stability studies, and it was shown that the material can be held at +18°C safely for up to 1 year. To guarantee stability of the CRM over its lifetime the stock will be maintained at −20°C. The results of the homogeneity and stability testing show that CRM–FDMT1 is appropriate for its intended use in quality assurance and quality control of shellfish toxin analysis methods. |
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