FURTHER CHARACTERIZATION OF MONOCLONAL ANTIBODY INDICATES SPECIFICITY FOR (6–4)-DIPYRIMIDINE PHOTOPRODUCTS |
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Authors: | P. T. Strickland O. Nikaioo T. Matsunaga J. M. Boyle |
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Affiliation: | Johns Hopkins School of Hygiene and Public Health, Baltimore, MD 21205, USA;Division of Radiation Biology, Kanazawa University, Kanazawa 920, Japan;Paterson Institute for Cancer Research, Manchester M20 9BX, UK |
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Abstract: | Abstract Monoclonal antibody aUVssDNA-1 is produced by hybridoma cell line 25JF.C3B6 originally selected from cell fusions using spleen cells from mice immunized with UV-irradiated polydeoxynucleotides (Strickland and Boyle, Photochem. Photobiol. 34 , 595–601, 1981). Original and subsequent studies of the binding characteristics of aUVssDNA-1 indicated that it was specific for cyclobuta-dithymidine photoproducts. Those investigations examined action spectrum, short-wavelength photo-reversal, nucleotide sequence effects, and photoreactivation using E. coli photolyase and incandescent tight. However, the more recent studies reported here examined acetophenone-UV-B photosensitization, UV-B photoisomerization, and photoreactivation using cloned E. coli photolyase and filtered incandescent light. The results indicate that aUVssDNA-1 recognizes photoproducts with characteristics of (6–4)-dipyrimidines. Thus, previous studies in which relatively rapid repair of cyclobuta-dithymidine photoproducts was inferred using this antibody, require re-interpretation in light of these new findings. |
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