Electrospun Highly Ordered Mesoporous Silica–Carbon Composite Nanofibers for Rapid Extraction and Prefractionation of Endogenous Peptides |
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Authors: | Gang‐Tian Zhu Dr. Xi Chen Xiao‐Mei He Han Wang Zheng Zhang Prof. Dr. Yu‐Qi Feng |
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Affiliation: | 1. Key Laboratory of Analytical Chemistry for Biology and Medicine (Ministry of Education), Department of Chemistry, Wuhan University, Wuhan 430072 (P.R. China);2. Wuhan Institute of Biotechnology, Wuhan 430072 (China) |
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Abstract: | A simple method was developed for the preparation of ordered mesoporous silica–carbon composite nanofibers (OMSCFs). The OMSCFs exhibited high carbon content, continuously long fibrous properties, uniform accessible mesopores, and a large surface area. The OMSCFs were also found to have ion‐exchange capacity. On the basis of the size‐exclusion effect of the mesopores and mixed‐mode hydrophobic/ion‐exchange interactions, the OMSCFs were applied for rapid enrichment of endogenous peptides by using a miniaturized solid‐phase extraction format. The adsorption mechanism was studied, and the eluting solution was optimized with standard peptide/protein solutions and protein digests. Employing a successive three‐step elution strategy, followed by LC‐MS/MS analysis, led to excellent performance with this approach in the extraction and prefractionation of peptides from human serum. |
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Keywords: | electrospinning ion exchange mesoporous materials nanofibers peptides |
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