Photophysical properties of Zn-substituted cytochrome c investigated by single-molecule and ensemble-averaged spectroscopy |
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Authors: | Choi Jungkweon Tachikawa Takashi Kim Youngmin Fujitsuka Mamoru Ihee Hyotcherl Majima Tetsuro |
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Affiliation: | The Institute of Scientific and Industrial Research (SANKEN), Osaka University, Mihogaoka 8-1, Ibaraki, Osaka 567-0047, Japan. |
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Abstract: | The study of the structural reorganization and photophysical properties of Zn-Cytc using the single-molecule and ensemble-averaged spectroscopy shows that the photoblinking behaviors of single-Zn-Cytc depend on the folded and unfolded structures, whereas the fluorescence dynamics of Zn-Cytc observed in the bulk phase are hardly affected by the conformational change of a protein. |
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