Polyamine concentration, transglutaminase activity and changes in protein synthesis during cryopreservation of shoot tips of apple variety Annurca |
| |
Authors: | Forni Cinzia Braglia Roberto Beninati Simone Lentini Alessandro Ronci Maurizio Urbani Andrea Provenzano Bruno Frattarelli Andrea Tabolacci Claudio Damiano Carmine |
| |
Affiliation: | Department of Biology, University of Rome Tor Vergata, Via della Ricerca Scientifica, Rome, Italy. forni@uniroma2.it |
| |
Abstract: | Changes in metabolism and protein expression were analysed during cryopreservation of the ancient apple variety Annurca. Our experiments concerned transglutaminase activity, polyamine levels and protein expression associated with shoot tip dehydration. Cryopreserved shoot tips displayed 72% regrowth after treatment in liquid medium with 0.75 M sucrose for 1 day followed by dehydration to 19% moisture content (fresh weight basis). After dehydration, the concentration of polyamines putrescine and spermidine decreased compared with untreated controls, while spermine concentration remained unaffected. Transglutaminase activity was slightly reduced in treated samples, while post-thaw regrowth enzyme activity approached control values. We also detected significant changes in protein expression profiles and identified six proteins related with stress response or involved in the slowing down of the cell cycle. The relationship between biochemical parameters, protein synthesis and cryotolerance is discussed. |
| |
Keywords: | |
本文献已被 PubMed 等数据库收录! |
|