| 基因芯片荧光图象采集与分析 |
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| 引用本文： | 胡茂海,陶纯堪,高万荣,杨晓春,袁祥岩. 基因芯片荧光图象采集与分析[J]. 光子学报, 2002, 31(5): 546-549 |
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| 作者姓名： | 胡茂海 陶纯堪 高万荣 杨晓春 袁祥岩 |
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| 作者单位： | 南京理工大学电光学院,210094 |
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| 摘 要： | 描述了基因芯片荧光图象的光学共聚焦成象,大范围高速扫描与控制,数据采集的原理和方法.并讨论了基因芯片荧光图象的分析算法,即模板定位与阈值分割相结合的半自动算法,并将其计算结果与完全采用人工分割算法的结果进行了比较.
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| 关 键 词： | 荧光显微成象 芯片分析 数据采集 扫描控制 |
| 收稿时间： | 2001-09-10 |
| 修稿时间： | 2001-09-10 |
COLLECTION AND ANALYSIS OF GENE-CHIP FLUORESCENT IMAGE |
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| Hu Maohai,Tao Chunkan,Gao Wanrong,Yang Xiaochun,Yuan Xiangyan Nanjing University of Science and Technology,Nanjing. COLLECTION AND ANALYSIS OF GENE-CHIP FLUORESCENT IMAGE[J]. Acta Photonica Sinica, 2002, 31(5): 546-549 |
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| Authors: | Hu Maohai Tao Chunkan Gao Wanrong Yang Xiaochun Yuan Xiangyan Nanjing University of Science Technology Nanjing |
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| Affiliation: | Hu Maohai,Tao Chunkan,Gao Wanrong,Yang Xiaochun,Yuan Xiangyan Nanjing University of Science and Technology,Nanjing 210094 |
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| Abstract: | It discusses in detail the principles and methods for gene chip fluorescent microscopy image,including confocal optics imaging,large scale scanning control as well as date collecting.The half automation segmentation algorithm with template locating and threshold segmenting for Gene chip fluorescent image is also analyzed.The calculated results are compared with the one obtained with manpower. |
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| Keywords: | Fluorescent microscopy imaging Chip analysis Data collecting Scanning control |
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