Contribution of solid-phase hexapeptide ligand libraries to the repertoire of human bile proteins |
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Authors: | Guerrier L Claverol S Finzi L Paye F Fortis F Boschetti E Housset C |
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Institution: | Bio-Rad Laboratories, C/o CEA-Saclay, Gif-sur-Yvette, France. |
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Abstract: | Proteins in bile may have important physiological functions and serve as disease biomarkers. Here, the protein composition of human gallbladder bile was analyzed using a recently described chromatography-like technology capable to enhance the signal of low-abundance species. First, proteins present in bile fluid were treated with immobilized peptide ligand libraries to concentrate dilute and very dilute species while concomitantly diluting the high-abundance proteins. The analysis of resulting protein mixture was then performed using LC-MS/MS after having classically separated proteins by a mini preparative gel electrophoresis. Overall 222 gene products were found; 143 of them were not reported before in proteomics studies. Ligand libraries by themselves contributed to find 81 new gene products distributed throughout different categories. The described chromatographic approach provides a significant contribution to the bile protein repertoire and opens new perspectives for the discovery of markers for specific biliary tract diseases. |
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