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Systemic Nuclear Proteomics Researches on Change of Jurkat T Lymphocyte Cells Under Radiation |
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| Authors: | LIANG Feng LIU Zhong-ying LIU Ning SONG Feng-rui ZHANG Xuan and LIU Zhi-qiang |
| Institution: | 1. College of Pharmacy, Jilin University, Changchun 130021, P. R. China; 2. Changchun Center of Mass Spectrometry, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun 130022, P. R. China; 3. Research Center for Genomic Medicine and MH Radiobiology Research Unit, School of Public Health, Jilin University, Changchun 130021, P. R. China |
| Abstract: | Radiation causes severe constraint on numerous pathological functions of cells, such as cell growth, nuclear genetic material expression and cell functions. In this study, we performed proteomic profiling of a nuclear fraction of Jurkat T lymphocyte cells under radiation along different time course by means of 2DE and MALDI TOF-MS. We found 24 protein spots whose expression had changed after radiation, including relevant proteins, genetic material proteins, metabolism proteins, molecular chaperon and nuclear membrane proteins. Based on the above it is concluded that the combination of fluorescence labeled 2D-PAGE and MALDI-TOF MS is more precisely and effectively to elucidate the protein changes in Jurkat T lymphocyte cells after irradiation. |
| Keywords: | Nuclear proteome Two-dimensional difference gel electrophoresis MALDI-TOF-MS Jurkat cell |
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