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Observation of subcellular structures of neurons by an illumination mode near-field optical microscope under an optical feedback control |
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| Authors: | Rajagopalan Uma Maheswari Shuji Mononobe Hitoshi Tatsumi Yoshifumi Katayama Motoichi Ohtsu |
| Institution: | 1. Kanagawa Academy of Science and Technology, KSP East 408, 3-2-1, Sakado, Takatsu-ku, Kawasaki, 213, Kanagazva, Japan 2. Department of Autonomic Physiology, Medical Research Institute, Tokyo Medical and Dental University, 101, Chiyoda-ku, Tokyo, Japan 3. “The Intelligence and its Origin,”, PRESTO, Japan Science and Technology Corporation, Japan 4. Interdisciplinary Graduate School of Science and Engineering, Tokyo Institute of Technology, 4259, Nagatsuda, Midori-ku, Yokohama, 226, Kanagazva, Japan |
| Abstract: | We propose an illumination mode near-field optical microscope operated under an optical feedback for imaging biological specimens in their natural environment. For feedback control, rapidly varying evanescent signal has been generated over the sample surface. It has been found that evanescent signal can be profitably used as a control signal by utilizing its sample feature dependent discrimination sensitivity. Neurons have been successfully observed both in air and in liquid with a resolution well beyond the diffraction limit. |
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