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In vivo microdialysis determination of collagen-induced serotonin release in rat blood |
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| Authors: | Tung-Hu Tsai Chieh-Fu Chen |
| Affiliation: | a Department of Pharmacology, National Research Institute of Chinese Medicine, Taipei 11221, Taiwan b Department of Pharmacology. National Yang-Ming University, Taipei 11221, Taiwan |
| Abstract: | We developed a sensitive microbore HPLC method coupled with an on-line microdialysis system to simultaneously measure endogenous 5-hydroxytryptamine (serotonin; 5-HT) and its major metabolite 5-hydroxyindoleacetic acid (5-HIAA) in the rat blood in vivo. A dialysis tube was placed in the right jugular vein. The validity of the procedure is demonstrated because analysis of the aggregating agents, collagen (I mg/kg) plus epinephrine (0.3 mg/kg) after intravenous injection, showed that they induced an increase in 5-HT and 5-HIAA levels in the jugular vein of the rat. |
| Keywords: | Microdialysis Serotonin 5-Hydroxyindoleacetic acid Collagen Epinephrine |
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