Analysis of DNA in Single Pollen Cell by Hadamard Transform Microscopic Fluorescence Imaging |
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| Authors: | Tang Hongwu Chen Guanquan Zeng Yun'e |
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| Affiliation: | Department of Analysis and Measurement Science, Wuhan University, Wuhan, 430072, People's Republic of China |
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| Abstract: | A noncommercial Hadamard transform microscopic image analysis system was used to measure fluorescence emitted by single cells. A method for subtracting the native fluorescence of theZephyranthes candida(Lindl.) Herb. pollen cell was developed based on its correlation to pollen area. Acridine orange and ethidium bromide were used as fluorescence probes for pollen DNA quantitative analysis, and the results obtained by the two methods were compared. |
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