| Molt-4细胞内游离镁离子浓度的31P NMR测定 |
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| 引用本文： | 黄荣清,杜泽涵.Molt-4细胞内游离镁离子浓度的31P NMR测定[J].波谱学杂志,1996,13(5):429-433. |
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| 作者姓名： | 黄荣清 杜泽涵 |
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| 作者单位： | 1. 军事医学科学院放射医学研究所, 北京 100850;2. 国家生物医学分析中心, 北京 100850 |
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| 摘 要： | 通过测量人淋巴瘤白血病细胞MOlt-4的31P谱中ATP的α磷和β磷的化学位移差值,得出细胞内Mg2+与ATP结合的复合物MgATP和整个ATP量的比值,计算得到Molt-4细胞内游离Mg2+浓度为0.258mmol/L.与其它分析方法相比,31P NMR测定细胞内游离Mg2+浓度具有对细胞样品无损伤的优点.
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| 关 键 词： | 游离Mg2+ 31P NMR MOlt-4细胞 |
| 收稿时间： | 1995-12-08 |
THE DETERMINATION OF CYTOSOLIC FREE MAGNESIUM ION CONCENTRATION IN HUMAN LYPHOMA CELL LINE Molt-4 BY USING 31P NMR |
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| Huang Rongqing,Du Zehan,Yan Xianzhong,Li Guangyu,Feng Rui.THE DETERMINATION OF CYTOSOLIC FREE MAGNESIUM ION CONCENTRATION IN HUMAN LYPHOMA CELL LINE Molt-4 BY USING 31P NMR[J].Chinese Journal of Magnetic Resonance,1996,13(5):429-433. |
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| Authors: | Huang Rongqing Du Zehan Yan Xianzhong Li Guangyu Feng Rui |
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| Institution: | Institute of Radiation Medicine, National centre of Biomedical Analysis, Academy of Military Medical Science, Beijing 100850 |
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| Abstract: | Based upon a measurement of chemical shift differences between the α phosphoryl and β phosphoryl resonances in the 31P spectrum of intracellular ATP in human lyphoma cell line Molt-4, the fraction of total ATP that is complexed to Mg2+ was estimated. Cytosolic free magnesium ion concentration in Molt-4 cells was determined noninvasively to be 0.258 mmol/L. |
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| Keywords: | Free Mg2+ 31P NMR Molt-4 cells |
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