标本溶血对SD大鼠14项生化检测结果的影响与分析

目的探讨标本溶血对SD大鼠14项生化检测结果的影响。方法采用TMS-1024全自动生化分析仪对20只大鼠不同溶血程度即Hb含量为0、12、、35、、10g/L的血清样本进行14项生化指标检测,并对结果进行统计学分析。结果溶血引起ALT、AST、TP、LDH、CK、TBi、UREA检测结果升高(P<0.05/0.01);引起CR、TG、A/G检测结果的降低(P<0.05/0.01);对GLU、CHO、ALP、ALB检测结果的影响不明显(P>0.05)。结论样本溶血可对较多的生化指标产生明显的影响,并且随溶血程度的增加,产生的影响越明显,其中以LDH、TG和AST最为敏感。

Influence of Hemolysis on Fourteen Biochemical Items in SD Rat Blood

Objective To investigate the influence of hemolysis on 14 biochemical items in SD rat blood.Methods Blood samples were collected from 20 SD rats,and using the TMS-1024 auto-biochemical analyzer,fourteen serum biochemical items were detected from each sample based on the different Hb contents,0,1,2,3,5,and 10 g/L.Results Hemolysis resulted in increasing of ALT,AST,TP,LDH,CK,TBi,and UREA levels(P<0.05/0.01) and decreasing of CR,TG,and A/G levels(P<0.05/0.01) in sera.But the levels of GLU,CHO,ALP,and ALB were not significantly affected by hemolysis(P>0.05).Conclusion A lot of biochemical items were significantly affected by hemolysis,and the levels of LDH,TG and AST are the more severely affected than others.