An investigation of microbore reverse-phase columns containing 3 μm packing materials |
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Authors: | N. K. Vadukul C. R. Loscombe |
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Abstract: | A procedure for packing 15 cm × 1 mm id reverse-phase microbore columns with 3 μm silicas obtained from different manufacturers is described. The speed of analysis and detection limits are compared to those obtained with a 50 cm × 1 mm id column packed with 10 μm ODS. The effect of detector time constant on the system, and flow rates on column efficiency are also examined. |
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Keywords: | Liquid chromatography, HPLC Microbore columns Reverse-phase mode 3 μ m packing materials |
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